survey data procedures version 17 0 20 Search Results


iscript cdna synthesis kit  (Bio-Rad)
99
Bio-Rad iscript cdna synthesis kit
Iscript Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tbs t solution  (Bio-Rad)
99
Bio-Rad tbs t solution
Tbs T Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horseradish peroxidase conjugated goat anti mouse igg  (Bio-Rad)
96
Bio-Rad horseradish peroxidase conjugated goat anti mouse igg
Horseradish Peroxidase Conjugated Goat Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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excimer laser nidek ec-5000  (Nidek CO)
90
Nidek CO excimer laser nidek ec-5000
Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an <t>excimer</t> laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.
Excimer Laser Nidek Ec 5000, supplied by Nidek CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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interleukin 4  (Miltenyi Biotec)
93
Miltenyi Biotec interleukin 4
Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an <t>excimer</t> laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.
Interleukin 4, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microwave decomposition system (mars 6  (CEM Corporation)
90
CEM Corporation microwave decomposition system (mars 6
Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an <t>excimer</t> laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.
Microwave Decomposition System (Mars 6, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
microwave decomposition system (mars 6 - by Bioz Stars, 2026-08
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microbanktm cryobeads  (Pro-Lab Diagnostics)
97
Pro-Lab Diagnostics microbanktm cryobeads
Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an <t>excimer</t> laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.
Microbanktm Cryobeads, supplied by Pro-Lab Diagnostics, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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loose sieved black peat soil graberde  (Plantaflor Humus Verkaufs GmbH)
90
Plantaflor Humus Verkaufs GmbH loose sieved black peat soil graberde
Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an <t>excimer</t> laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.
Loose Sieved Black Peat Soil Graberde, supplied by Plantaflor Humus Verkaufs GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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x tdt buffer  (New England Biolabs)
98
New England Biolabs x tdt buffer
Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an <t>excimer</t> laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.
X Tdt Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pi 17 0 20 4  (Avanti Polar)
93
Avanti Polar pi 17 0 20 4
Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an <t>excimer</t> laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.
Pi 17 0 20 4, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anton paar rxa 170 analyzer  (Anton Paar)
90
Anton Paar anton paar rxa 170 analyzer
Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an <t>excimer</t> laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.
Anton Paar Rxa 170 Analyzer, supplied by Anton Paar, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad slot blot apparatus  (Bio-Rad)
99
Bio-Rad bio rad slot blot apparatus
Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an <t>excimer</t> laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.
Bio Rad Slot Blot Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an excimer laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.

Journal: Investigative Ophthalmology & Visual Science

Article Title: Mice Deficient in TAZ (Wwtr1) Demonstrate Clinical Features of Late-Onset Fuchs’ Endothelial Corneal Dystrophy

doi: 10.1167/iovs.64.4.22

Figure Lengend Snippet: Wwtr1 deficient mice demonstrated impaired corneal endothelial wound healing after phototherapeutic keratectomy (PTK). An endothelial wound was created with an excimer laser (20 µm depth) in 6-month-old WT ( n = 6) and Wwtr1 +/− ( n = 6) mice. Optical coherence tomography (OCT) ( A, B ), slit lamp biomicroscopy ( C ), and in vivo confocal microscopy (IVCM) ( D ) were performed on post-injury days 1, 3, 5, 7, 10, and 14. Central corneal thickness (CCT) measured with OCT did not show statistical difference between the two genotypes ( B ), however, CCT reached its maximal thickness on post-injury days (PIDs) 7 to 10 and corneal edema was more marked on PID 10 in Wwtr1 +/− mice ( C ). IVCM revealed the regenerating course of injured endothelial cells throughout the study period ( D ). Following euthanasia on PID 14, endothelial wholemounts were stained with alizarin red ( E ), and endothelial cell density (ECD) was measured. ECD was significantly reduced in Wwtr1 +/− mice versus WT, suggesting impaired endothelial healing in Wwtr1 deficient mice. CCT was analyzed using a 2-way ANOVA, ECD was analyzed using a Mann-Whitney U test, * P < 0.05. OCT scale bars equivalent to 250 µm, IVCM and Alizarin red microscopy scale bars equivalent to 50 µm.

Article Snippet: Mice were sedated, then a drop of proparacaine was applied to anesthetize the cornea and an excimer laser injury (Nidek EC-5000 Excimer Laser, average fluence of 170 mK/cm 2 with an ablation zone diameter of 2 mm and depth of 20 μm) was performed.

Techniques: Tomography, In Vivo, Confocal Microscopy, Staining, MANN-WHITNEY, Microscopy